TSH ELISA Kit

Produktnummer ABIN6959968

Produktdetails TSH ELISA Kit

Target: TSH (Thyroid Stimulating Hormone (TSH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.3 mIU/L - 12.0 mIU/L

Untere Nachweisgrenze: 0.3 mIU/L

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TSH in human serum.

Proben: Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Thyroid Stimulating Hormone (TSH)

Sensitivität: 0.14 mIU/L

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use.
2. Standard - The concentration of the standard is as following: 12.0mIU/L, 6.0mIU/L, 3.0mIU/L, 1.5mIU/L, 0.6mIU/L, 0.3mIU/L.
3. Wash Solution - Dilute 15 mL of Wash Solution concentrate (20x) with 285 mL of deionized or distilled water to prepare 300 mL of Wash Solution (1x).
4. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. If crystals have formed in the Wash Solution concentrate (20x), warm to room temperature and mix gently until the crystals are completely dissolved.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Haltbarkeit: 6 months

Referenzen für TSH ELISA Kit (ABIN6959968)

Le Stunff, Tilotta, Sadoine, Le Denmat, Briet, Motte, Clauser, Bougnères, Chaussain, Silve: "Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 32, Issue 2, pp. 333-346, (2016) (PubMed).

Details zu TSH

Target: TSH (Thyroid Stimulating Hormone (TSH))

Abstract: TSH Produkte

Synonyme: CG7665 ELISA Kit, DLGR-1 ELISA Kit, DLGR1 ELISA Kit, Dmel\CG7665 ELISA Kit, FSH ELISA Kit, FSH-TSH ELISA Kit, Fsh ELISA Kit, Fsh/CG7665 ELISA Kit, LGR1 ELISA Kit, LH/CG receptor ELISA Kit, RH44949p ELISA Kit, TSH ELISA Kit, dLGR1 ELISA Kit, Leucine-rich repeat-containing G protein-coupled receptor 1 ELISA Kit, Lgr1 ELISA Kit

Substanzklasse: Hormone