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TGFB2 ELISA Kit

TGFB2 Reaktivität: Human Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN6960089
  • Target Alle TGFB2 ELISA Kits anzeigen
    TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
    Reaktivität
    • 9
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    24.69 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    24.69 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of TGFb2 in human serum, plasma, tissue homogenates, cell culture supernates.
    Proben
    Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 2 (TGFb2)
    Sensitivität
    9.17 pg/mL
    Bestandteile
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Kommentare

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Probenmenge
    50 μL
    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      Then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    Aufbereitung der Reagenzien
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 6,000pg/mL. Please firstly dilute the stock solution to 2,000pg/mL and the diluted standard serves as the highest standard (2,000pg/mL). Then prepare 5 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 2,000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.69pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
    4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    Aufbereitung der Proben
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Vorsichtsmaßnahmen
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    .
    Haltbarkeit
    6 months
  • Li, Li, Sun, Peng, Zhao, Zhong et al.: "Influence of the Expression Level of O6-Alkylguanine-DNA Alkyltransferase on the Formation of DNA Interstrand Crosslinks Induced by Chloroethylnitrosoureas in Cells: A Quantitation Using ..." in: PLoS ONE, Vol. 10, Issue 3, pp. e0121225, (2015) (PubMed).

  • Target Alle TGFB2 ELISA Kits anzeigen
    TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))
    Andere Bezeichnung
    Transforming Growth Factor Beta 2 (TGFb2) (TGFB2 Produkte)
    Synonyme
    LDS4 ELISA Kit, TGF-beta2 ELISA Kit, tgf-beta2 ELISA Kit, tgfb2-A ELISA Kit, BB105277 ELISA Kit, Tgf-beta2 ELISA Kit, Tgfb-2 ELISA Kit, TGF-beta 2 ELISA Kit, Tgfbr2T ELISA Kit, TGFB2 ELISA Kit, TGFbeta2 ELISA Kit, MGF ELISA Kit, TGF-B2 ELISA Kit, transforming growth factor beta 2 ELISA Kit, transforming growth factor beta 2 L homeolog ELISA Kit, transforming growth factor, beta 2 ELISA Kit, transforming growth factor, beta receptor 2 ELISA Kit, TGFB2 ELISA Kit, tgfb2.L ELISA Kit, Tgfb2 ELISA Kit, Tgfbr2 ELISA Kit, tgfb2 ELISA Kit
    Pathways
    Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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