IL12 ELISA Kit

Produktnummer ABIN6963619

Produktdetails IL12 ELISA Kit

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.63 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.63 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This kit recognizes Mouse IL-12 in samples. No Significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.

Sensitivität: 9.38 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Aufbereitung der Reagenzien:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 1000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 1000, 500, 250, 125, 62.5, 31.25, 15.63, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 1000 pg/mL working solution to the first tube and mix up to produce a 500 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-12 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Haltbarkeit: 12 months

Referenzen für IL12 ELISA Kit (ABIN6963619)

Amin, Bughdadi, Abo-Zaid, Ismail, El-Agamy, Alqahtani, El-Sayyad, Rezk, Ramadan: "Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress." in: Journal of food biochemistry, Vol. 43, Issue 12, pp. e13050, (2020) (PubMed).

Li, Kong, Chen, Tang, Jin, Yan, Wang: "Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis." in: Oxidative medicine and cellular longevity, Vol. 2019, pp. 1475729, (2020) (PubMed).

Kim, Seong, Choung: "Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function." in: Nutrients, Vol. 12, Issue 1, (2020) (PubMed).

Li, Huang, Yang, Kan, Yao, Liu, Pu, He, Khan, Qi, Zhou, Shu, Chen: "Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway." in: Scientific reports, Vol. 10, Issue 1, pp. 9868, (2020) (PubMed).

Santos, Mazzoli, Maia, Saggese, Isticato, Leite, Iossa, Ricca, Baccigalupi: "A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC." in: Microbial cell factories, Vol. 19, Issue 1, pp. 42, (2020) (PubMed).

García-Bernal, Palomo, Martínez, Millán-Rivero, García-Guillén, Blanquer, Díaz-Ricart, Sackstein, Carreras, Moraleda: "Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease." in: Journal of cellular and molecular medicine, Vol. 24, Issue 14, pp. 8031-8044, (2020) (PubMed).

Tang, Sui, Liu, Zhang, Liu, Wang, Zhao, Sun, Liu, Luo, Lai, Liu, Deng, Song: "Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages - A promising cancer immunotherapy." in: International journal of pharmaceutics, Vol. 590, pp. 119929, (2020) (PubMed).

Sun, Li, Xiao, Huang, Wang, Huang, Yin, Liu, Xu, Wei: "Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection." in: Microbial pathogenesis, Vol. 147, pp. 104441, (2020) (PubMed).

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: Interleukin 12 (IL12 Produkte)

Synonyme: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, LOC100217488 ELISA Kit, IL-12p35 ELISA Kit, Il-12a ELISA Kit, Ll12a ELISA Kit, p35 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, IL-12 p35 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12A ELISA Kit, interleukin 12b ELISA Kit, interleukin 12a ELISA Kit, Interleukin 12 level ELISA Kit, IL12B ELISA Kit, IL12A ELISA Kit, Il12b ELISA Kit, Il12a ELISA Kit, IL12 ELISA Kit, il12a ELISA Kit

Hintergrund: IL12, IL-12 p70, IL12 p70

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome