HSP90 ELISA Kit

Produktnummer ABIN6999415

Produktdetails HSP90 ELISA Kit

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Verwendungszweck: The kit is a high sensitivity sandwich enzyme immunoassay for in vitro quantitative measurement in various sample types.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90.

Sensitivität: 28 pg/mL

Güteklasse: High Sensitivity

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Probenmenge: 100 µL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit is not used up all at once, remove only the strips and reagents for the current experiment and leave the remaining strips and reagents in the desired condition.
2. Standard - Reconstitute the standard with the Standard Diluent, keep it at room temperature for 10 minutes and shake it gently (do not let it foam). Please prepare tubes with Standard Diluent and make a dilution series. Mix each tube thoroughly before the next transfer. The last tube with Standard Diluent is the blank as 0 mg/mL.
3. Detection Reagent A and Detection Reagent B - Spin or centrifuge the stock of Detection Reagent A and B briefly before use. Dilute to working concentration (1:100) with Assay Diluent A or B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution Concentrate (30x) with 580 mL of deionized or distilled water to make 600 mL of Wash Solution (1x).
5. TMB Substrate - Aspirate the required amount of solution with sterile tip and do not return the residual solution back into the vial.

Note:

1. Serial dilution directly in the wells is not recommended.
2. Prepare standard within 15 minutes before assay. Do not dissolve the reagents directly at 37 °C.
3. Detection Reagent A and B are sticky solutions, so pipette them slowly to reduce volume errors.
4. Reconstitute Standard or working solutions of Detection Reagent A and B carefully according to instructions, avoiding foaming and mixing gently until crystals are completely dissolved. To minimize inaccuracy caused by pipetting, use small volumes and ensure pipettes are calibrated. It is recommended to aspirate more than 10 µL for one-time pipetting.
5. The reconstituted Standard, Detection Reagent A and B can only be used once.
6. When crystals have formed in the Wash Solution concentrate (30x), warm it to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or preparation containers affect the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 ° C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

Haltbarkeit: 6 months

Details zu HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Andere Bezeichnung: Heat Shock Protein 90 (HSP90 Produkte)

Synonyme: EL52 ELISA Kit, HSP86 ELISA Kit, HSP89A ELISA Kit, HSP90A ELISA Kit, HSP90N ELISA Kit, HSPC1 ELISA Kit, HSPCA ELISA Kit, HSPCAL1 ELISA Kit, HSPCAL4 ELISA Kit, HSPN ELISA Kit, Hsp89 ELISA Kit, Hsp90 ELISA Kit, LAP2 ELISA Kit, git10 ELISA Kit, swo1 ELISA Kit, HSP90 ELISA Kit, htpG ELISA Kit, SCBAC25F8.08 ELISA Kit, 23.m06066 ELISA Kit, 17.m07646 ELISA Kit, HSP90-1 ELISA Kit, 143198_at ELISA Kit, 83 ELISA Kit, 83K HSP ELISA Kit, DMHSP82 ELISA Kit, E(sev)3A ELISA Kit, E(sina)2 ELISA Kit, HSP82 ELISA Kit, HSP83 ELISA Kit, ORF1 ELISA Kit, Su(Raf)3A ELISA Kit, anon-EST:Liang-2.53 ELISA Kit, anon-WO0068693 ELISA Kit, anon-WO0140519.209 ELISA Kit, clone 2.53 ELISA Kit, en(lz)3C/4C ELISA Kit, hsp84 ELISA Kit, l(3)j5C2 ELISA Kit, ms(3)08445 ELISA Kit, stc ELISA Kit, DmelCG1242 ELISA Kit, CG1242 ELISA Kit, Hsp86 ELISA Kit, Hspca ELISA Kit, 86kDa ELISA Kit, 89kDa ELISA Kit, AL024080 ELISA Kit, AL024147 ELISA Kit, Hsp86-1 ELISA Kit, hsp4 ELISA Kit, hsp86 ELISA Kit, hsp89 ELISA Kit, hsp90 ELISA Kit, hsp90a ELISA Kit, hspc1 ELISA Kit, hspca ELISA Kit, hspn ELISA Kit, lap2 ELISA Kit, Hsp90alpha ELISA Kit, heat shock protein 90 alpha family class A member 1 ELISA Kit, heat shock protein Hsp90 ELISA Kit, Hsp90 chaperone ELISA Kit, Heat shock protein 90 ELISA Kit, heat shock protein 90 ELISA Kit, chaperone protein HtpG ELISA Kit, molecular chaperone HtpG ELISA Kit, Heat Shock Protein 90 ELISA Kit, uncharacterized LOC100384473 ELISA Kit, Heat shock protein 83 ELISA Kit, heat shock protein 90, alpha (cytosolic), class A member 1 ELISA Kit, heat shock protein 90 alpha family class B member 1 ELISA Kit, heat shock protein 90kDa alpha family class A member 1 L homeolog ELISA Kit, heat shock protein HSP 90-alpha ELISA Kit, HSP90AA1 ELISA Kit, hsp90 ELISA Kit, HSP90 ELISA Kit, daf-21 ELISA Kit, htpG ELISA Kit, SCO7516 ELISA Kit, MAP_RS10510 ELISA Kit, TP04_0646 ELISA Kit, TP01_0934 ELISA Kit, APH_RS03525 ELISA Kit, GbCGDNIH1_0315 ELISA Kit, MAV_2118 ELISA Kit, HSP90C ELISA Kit, BBOV_IV008400 ELISA Kit, BBOV_III007380 ELISA Kit, ACICU_00312 ELISA Kit, ECL_01244 ELISA Kit, YE105_C1172 ELISA Kit, pco153543(105) ELISA Kit, Hsp83 ELISA Kit, Hsp90aa1 ELISA Kit, HSP90AB1 ELISA Kit, hsp90aa1.1.L ELISA Kit, LOC108698781 ELISA Kit

Hintergrund: HtpG, Hsp-90 NTD, N-Terminal Domain

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals