Factor VII ELISA Kit

Produktnummer ABIN6999452

Produktdetails Factor VII ELISA Kit

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Verwendungszweck: The kit is a high sensitivity sandwich enzyme immunoassay for in vitro quantitative measurement in various sample types.

Proben: Plasma

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VII.

Sensitivität: 0.28 ng/mL

Güteklasse: High Sensitivity

Bestandteile:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Anwendungsinformationen

Probenmenge: 100 µL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Aufbereitung der Reagenzien:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit is not used up all at once, remove only the strips and reagents for the current experiment and leave the remaining strips and reagents in the desired condition.
2. Standard - Reconstitute the Standard with 1.0mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 400ng/mL. Firstly dilute the stock solution to 50ng/mL and the diluted standard serves as the highest standard (50ng/mL). Then prepare 7 tubes containing 0.5mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.12ng/mL, 1.56ng/mL, 0.78ng/mL, and the last tubes with Standard Diluent is the blank as 0ng/mL.
3. Detection Reagent A and Detection Reagent B - Spin or centrifuge the stock of Detection Reagent A and B briefly before use. Dilute to working concentration (1:100) with Assay Diluent A or B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution Concentrate (30x) with 580 mL of deionized or distilled water to make 600 mL of Wash Solution (1x).
5. TMB Substrate - Aspirate the required amount of solution with sterile tip and do not return the residual solution back into the vial.

Note:

1. Serial dilution directly in the wells is not recommended.
2. Prepare standard within 15 minutes before assay. Do not dissolve the reagents directly at 37 °C.
3. Detection Reagent A and B are sticky solutions, so pipette them slowly to reduce volume errors.
4. Reconstitute Standard or working solutions of Detection Reagent A and B carefully according to instructions, avoiding foaming and mixing gently until crystals are completely dissolved. To minimize inaccuracy caused by pipetting, use small volumes and ensure pipettes are calibrated. It is recommended to aspirate more than 10 µL for one-time pipetting.
5. The reconstituted Standard, Detection Reagent A and B can only be used once.
6. When crystals have formed in the Wash Solution concentrate (30x), warm it to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or preparation containers affect the detection result.

Aufbereitung der Proben:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 ° C

Informationen zur Lagerung:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

Haltbarkeit: 6 months

Details zu Factor VII

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Andere Bezeichnung: Coagulation Factor VII (F7 Produkte)

Synonyme: F7 ELISA Kit, wu:fb59f04 ELISA Kit, zgc:109870 ELISA Kit, SPCA ELISA Kit, AI114908 ELISA Kit, ATIII ELISA Kit, At-3 ELISA Kit, At3 ELISA Kit, LOC100223776 ELISA Kit, AI132620 ELISA Kit, Cf7 ELISA Kit, FVII ELISA Kit, mfVII ELISA Kit, f7 ELISA Kit, coagulation factor VII ELISA Kit, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog ELISA Kit, F7 ELISA Kit, f7 ELISA Kit, Serpinc1 ELISA Kit, CpipJ_CPIJ009142 ELISA Kit, CpipJ_CPIJ010295 ELISA Kit, CpipJ_CPIJ020127 ELISA Kit, fa7 ELISA Kit, f7.S ELISA Kit

Hintergrund: FVII, SPCA, proconvertin, Cothromboplastin, Serum Prothrombin Conversation Accelerator, Stable Factor, Eptacog alfa

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling