Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

HCP ELISA Kit

HCP Reaktivität: Chinese Hamster Colorimetric Sandwich ELISA 3.125 ng/mL – 100 ng/mL Cell Culture Supernatant
Produktnummer ABIN7538037
  • Target Alle HCP Produkte
    HCP (Host Cell Proteins (HCP))
    Reaktivität
    • 1
    • 1
    • 1
    • 1
    Chinese Hamster
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    3.125 ng/mL – 100 ng/mL
    Untere Nachweisgrenze
    3.125 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.
    Proben
    Cell Culture Supernatant
    Analytische Methode
    Quantitative
    Kreuzreaktivität (Details)
    No cross-reaction exists between the homologous antigens.
    Sensitivität
    0.2 ng/mL
    Produktmerkmale
    This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.
    Bestandteile
    Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Probenmenge
    100 µL
    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Testpräzision
    Intra Variation%: 3.1-4.5, Inter Variation%: 6.0-9.1
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle HCP Produkte
    HCP (Host Cell Proteins (HCP))
    Andere Bezeichnung
    Host Cell Proteins (HCP Produkte)
Sie sind hier:
Kundenservice