HCP ELISA Kit

Produktnummer ABIN7538037

Produktdetails HCP ELISA Kit

Target: HCP (Host Cell Proteins (HCP))

Reaktivität: Chinese Hamster

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.125 ng/mL – 100 ng/mL

Untere Nachweisgrenze: 3.125 ng/mL

Applikation: ELISA

Verwendungszweck: The kit is intended for the quantitative determination of host cell protein impurities in Cell culture supernatants or host cell protein (HCP) contamination in end-product expressed by Chinese Hamster Ovary (CHO) expression systems.

Proben: Cell Culture Supernatant

Analytische Methode: Quantitative

Kreuzreaktivität (Details): No cross-reaction exists between the homologous antigens.

Sensitivität: 0.2 ng/mL

Produktmerkmale: This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Bestandteile: Pre-Coated Microplate (Detachable), Standard (Stock Solution), Detection antibody (100x), TMB Substrates, Stop Solution, Wash Solution (100x), Diluent Buffer (10x), Plate Sealer

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 µL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra Variation%: 3.1-4.5, Inter Variation%: 6.0-9.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu HCP

Target: HCP (Host Cell Proteins (HCP))

Andere Bezeichnung: Host Cell Proteins (HCP Produkte)