IL-5 ELISA Kit

Produktnummer ABIN921063

Produktdetails IL-5 ELISA Kit

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Bindungsspezifität: AA 20-134

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-5

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: sf21
Immunogen sequence: I20-S134

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: sf21
Immunogen sequence: I20-S134

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the IL-5 family.

Plattentyp: Pre-coated

Protokoll: human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-5 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human IL-5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 44.6, Standard deviation: 2.32, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 168, Standard deviation: 6.89, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 338, Standard deviation: 14.87, CV(%): 4.4,
• Sample 1: n=24, Mean(pg/ml): 43.8, Standard deviation: 2.63, CV(%): 6
• Sample 2: n=24, Mean(pg/ml): 188, Standard deviation: 10.34, CV(%): 5.5
• Sample 3: n=24, Mean(pg/ml): 329, Standard deviation: 21.06, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-5 ELISA Kit (ABIN921063)

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, (2013) (PubMed).

Chen, Duan, Kang, Yang, Jiang, Zhang, Li, Yin, Hu, Dong, Li, Hajjar, Han: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, (2012) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Tong, Bai, Liu, Wang, Zhang, Xiao, Xu, Liu, Yang, Wu: "Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18." in: Veterinary immunology and immunopathology, Vol. 136, Issue 3-4, pp. 194-200, (2010) (PubMed).

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL5 (IL5 Produkte)

Synonyme: EDF ELISA Kit, IL-5 ELISA Kit, TRF ELISA Kit, Il-5 ELISA Kit, IL5 ELISA Kit, interleukin 5 ELISA Kit, IL5 ELISA Kit, Il5 ELISA Kit

Hintergrund:

Protein Function: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells.

Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.

Synonyms: Interleukin-5,IL-5,B-cell differentiation factor I,Eosinophil differentiation factor,T-cell replacing factor,TRF,IL5,

Full Gene Name: Interleukin-5

Cellular Localisation: Secreted.

Gen-ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour