CCL2 ELISA Kit

Produktnummer ABIN921079

Produktdetails CCL2 ELISA Kit

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Bindungsspezifität: AA 24-96

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MCP-1

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: Q24-R96

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <6pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: Q24-R96

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse MCP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MCP-1 has been precoated onto 96-well plates. Standards(E.coli, Q24-R96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MCP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MCP-1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse MCP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MCP-1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 51, Standard deviation: 2.142, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 326, Standard deviation: 16.63, CV(%): 5.1
• Sample 3: n=16, Mean(pg/ml): 658, Standard deviation: 36.85, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 64, Standard deviation: 3.392, CV(%): 5.3
• Sample 2: n=24, Mean(pg/ml): 358, Standard deviation: 20.41, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 712, Standard deviation: 42.72, CV(%): 6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CCL2 ELISA Kit (ABIN921079)

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Details zu CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Andere Bezeichnung: CCL2 (CCL2 Produkte)

Synonyme: GDCF-2 ELISA Kit, HC11 ELISA Kit, HSMCR30 ELISA Kit, MCAF ELISA Kit, MCP-1 ELISA Kit, MCP1 ELISA Kit, SCYA2 ELISA Kit, SMC-CF ELISA Kit, AI323594 ELISA Kit, JE ELISA Kit, Scya2 ELISA Kit, Sigje ELISA Kit, MCP-1A ELISA Kit, MCP1A ELISA Kit, C-C motif chemokine ligand 2 ELISA Kit, chemokine (C-C motif) ligand 2 ELISA Kit, C-C motif chemokine 2 ELISA Kit, CCL2 ELISA Kit, Ccl2 ELISA Kit, LOC101120093 ELISA Kit

Hintergrund:

Protein Function: Chemotactic factor that attracts monocytes, but not neutrophils.

Background: Monocyte chemotactic protein-1(MCP-1) is a member of the small inducible gene(SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1(MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1(Q24-R96) with the molecular mass of 8.5Kda.

Synonyms: C-C motif chemokine 2,Monocyte chemoattractant protein 1,Monocyte chemotactic protein 1,MCP-1,Platelet-derived growth factor-inducible protein JE,Small-inducible cytokine A2,Ccl2,Je, Mcp1, Scya2,

Full Gene Name: C-C motif chemokine 2

Cellular Localisation: Secreted.

Gen-ID: 20296

UniProt: P10148

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion