CXCL10 ELISA Kit

Produktnummer ABIN921093

Dieses Human CXCL10 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CXCL10 zu quantifizieren. Dieses ELISA Kit wurde in 1 Publikation zitiert.

Kurzübersicht für CXCL10 ELISA Kit (ABIN921093)

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Bindungsspezifität: AA 22-98

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Produktdetails CXCL10 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL10/IP-10

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: V22-P98

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: V22-P98

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plattentyp: Pre-coated

Protokoll: human CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL10 has been precoated onto 96-well plates. Standards(E.coli, V22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL10 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL10 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 11.7, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 837, Standard deviation: 38.5, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 1469, Standard deviation: 89.6, CV(%): 6.1,
• Sample 1: n=24, Mean(pg/ml): 273, Standard deviation: 18.3, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 996, Standard deviation: 58.8, CV(%): 5.9
• Sample 3: n=24, Mean(pg/ml): 1478, Standard deviation: 115.3, CV(%): 7.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CXCL10 ELISA Kit (ABIN921093)

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Details zu CXCL10

Target: CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

Andere Bezeichnung: CXCL10

Hintergrund:

Protein Function: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3.

Background: Chemokine(C-X-C motif) ligand 10(CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein(gamma-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Synonyms: C-X-C motif chemokine 10,10 kDa interferon gamma-induced protein,Gamma-IP10,IP-10,Small-inducible cytokine B10,CXCL10(1-73),CXCL10,INP10, SCYB10,

Full Gene Name: C-X-C motif chemokine 10

Cellular Localisation: Secreted.

Gen-ID: 3627

UniProt: P02778