IL-7 ELISA Kit

Produktnummer ABIN921099

Produktdetails IL-7 ELISA Kit

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Bindungsspezifität: AA 26-154

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-7

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: E26-I154

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: E26-I154

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli, E26-I154) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-7 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-7 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 47.8, Standard deviation: 2.49, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 376, Standard deviation: 13.16, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 755, Standard deviation: 35.49, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 52.5, Standard deviation: 3.31, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 388, Standard deviation: 32.59, CV(%): 8.4
• Sample 3: n=24, Mean(pg/ml): 746, Standard deviation: 40.3, CV(%): 5.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-7 ELISA Kit (ABIN921099)

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL7 (IL7 Produkte)

Synonyme: IL7 ELISA Kit, IL-7 ELISA Kit, A630026I06Rik ELISA Kit, Il-7 ELISA Kit, hlb368 ELISA Kit, interleukin 7 ELISA Kit, IL7 ELISA Kit, Il7 ELISA Kit

Hintergrund:

Protein Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Background: Interleukin 7(IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7(IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17(T(H)17) cells in experimental autoimmune encephalomyelitis(EAE).2

Synonyms: Interleukin-7,IL-7,Il7,Il-7,

Full Gene Name: Interleukin-7

Cellular Localisation: Secreted.

Gen-ID: 16196

UniProt: P10168

Pathways: JAK-STAT Signalweg