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IgG2b ELISA Kit

Reaktivität: Maus Colorimetric Sandwich ELISA Media, Serum
Produktnummer ABIN930363
  • Target Alle IgG2b Produkte
    IgG2b
    Reaktivität
    • 3
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Proben
    Media, Serum
    Analytische Methode
    Quantitative
    Produktmerkmale
    ELISA kit for the detection of Mouse IgG2 b Isotyping in the research laboratory
    Alternative Names: Mouse IgG2b Isotyping ELISA kit
    Aufreinigung
    Protein A affinity column
  • Applikationshinweise
    Optimal working dilutions should be determined experimentally by the investigator.
    Kommentare

    Mouse IgG2b Isotyping ELISA kit

    Plattentyp
    Pre-coated
    Protokoll
    This mouse IgG Isotyping Enzyme Immunometric Assay (EIA) kit is a 2b complete kit for the determintation of mouse IgG in Tissue CultureMedia and serum. 2b Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG Standard is provided in the kit. After a simultaneous incubation with a polyclonal 2b antibody to antimouse IgG conjugated to Horseradish peroxidase, which binds to the mouse IgG 2b captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG in either standards or 2b samples. - See more at: https://www.fitzgerald-fii.com/mouse-igg2b-isotyping-elisa-kit-55r-ib09601.html#sthash.TRgGsoNm.dpuf
    Testdurchführung

    This mouse IgG Isotyping Enzyme Immunometric Assay (EIA) kit is a 2b complete kit for the determintation of mouse IgG in Tissue Culture Media and serum.
    Please read the complete kit insert before performing this assay.
    The kit uses a polyclonal antibody to mouse IgG immobilized on a microtiter plate to bind the mouse IgG in the standards or sample. A mouse IgG Standard is provided in the kit.
    After a simultaneous incubation with a polyclonal 2b antibody to antimouse IgG conjugated to Horseradish peroxidase, which binds to the mouse IgG 2b captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgG in either standards or 2b samples.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8 °C
  • Target Alle IgG2b Produkte
    IgG2b
    Abstract
    IgG2b Produkte
    Substanzklasse
    Antibody
    Hintergrund
    IgG2 is divided into two subclasses, IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a varying portion called the "hinge region" which is unique to each IgG. This region allows for molecular flexibility and sets IgG2a apart from its IgG counterparts. Properties of IgG2a include neutralization of toxins and diffusion in the extracellular space. Synonyms: Mouse IgG2b Isotyping ELISA kit.
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