Prostaglandin F2alpha ELISA Kit

Produktnummer ABIN930394

Produktdetails Prostaglandin F2alpha ELISA Kit

Target: Prostaglandin F2alpha

Reaktivität: Hormone

Methodentyp: Competition ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of PGF2 alpha in the research laboratory
Alternative Names: Prostaglandin F2 ELISA kit, PGF2 ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Testdurchführung:

This Prostaglandin F kit is a competitive immunoassay for the determination of Prostaglandin F in biological fluids. Please read the complete kit insert polyclonal antibody to PGF to bind, in a competitive manner, the PGF in the sample or an alkaline phosphatase molecule which has PGF covalently attached to it. After a simultaneous incubation at room temperature the excess reagents away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow coloris inversely proportional to the concentration of PGF in either standards or samples. The measured optical density is used to calculate the concentration of prostaglandin F2 alpha.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Prostaglandin F2alpha

Target: Prostaglandin F2alpha

Andere Bezeichnung: PGF2 alpha (Prostaglandin F2alpha Produkte)

Substanzklasse: Hormone

Hintergrund: Prostaglandin F2 alpha (PGF2a in prostanoid nomenclature) is a naturally occurring prostaglandin used in medicine to induce labor and as an abortifacient. In domestic mammals, it is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the follicular phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2 alpha is dependent on the number of receptors on the corpus luteum membrane. The PGF2a isoform 8isoPGF2a was found in significantly increased amounts in patients with endometriosis, thus being a potential causative link in endometriosis-associated oxidative stress.
Synonyms: Prostaglandin F2 ELISA kit, PGF2 ELISA kit.