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Substance P ELISA Kit

Reaktivität: Human Competition ELISA
Produktnummer ABIN930395
  • Target Alle Substance P ELISA Kits anzeigen
    Substance P
    Reaktivität
    • 13
    • 13
    • 10
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
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    Human
    Methodentyp
    Competition ELISA
    Applikation
    ELISA
    Produktmerkmale
    ELISA kit for the detection of Substance P in the research laboratory
    Alternative Names: Substance P ELISA kit
    Top Product
    Discover our top product Substance P ELISA Kit
  • Applikationshinweise
    Optimal conditions to be determined by end user
    Testdurchführung

    This Substance P kit is a competitive immunoassay for the determination of Substance P in biological fluids. Please read the complete kit insert before performing this assay. The kit for the measurement of Substance P uses a polyclonal antibody to Substance P to bind, in a competitive manner, the Substance P in the standard or sample or an alkalinephosphatase molecule which has Substance P covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of Substance P in either standards or samples. The measured optical density is used to calculate the concentration of Substance P.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8 °C.
  • Target Alle Substance P ELISA Kits anzeigen
    Substance P
    Abstract
    Substance P Produkte
    Hintergrund
    Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman's group in 1970. Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.
    Synonyms: Substance P ELISA kit.
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