hCG ELISA Kit

Produktnummer ABIN930402

Produktdetails hCG ELISA Kit

Target: hCG (Chorionic Gonadotropin (hCG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of hCG in the research laboratory
Alternative Names: hCG ELISA kit, human Chorionic Gonadotropin ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Plattentyp: Pre-coated

Testdurchführung:

The IBL hCG is a direct solid phase sandwich ELISA method. The samples and diluted anti hCGHRP conjugate are added to the wells coated with Mab to beta subunit. hCG in the patient's serum binds to antihCG MAb on the well and the antihCG second antibody then binds to hCG. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of hCG in the samples. A standard curve is prepared relating color intensity to the concentration of the hCG.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Referenzen für hCG ELISA Kit (ABIN930402)

Piché, Sauvageau, Vanlian, Erythropel, Robaire, Leask: "Effects of di-(2-ethylhexyl) phthalate and four of its metabolites on steroidogenesis in MA-10 cells." in: Ecotoxicology and environmental safety, Vol. 79, pp. 108-15, (2012) (PubMed).

Details zu hCG

Target: hCG (Chorionic Gonadotropin (hCG))

Andere Bezeichnung: hCG (hCG Produkte)

Substanzklasse: Hormone

Hintergrund: Human Chorionic Gonadotropin (hCG) is a 40 kD glycoprotein hormone secreted by the placenta. hCG has two subunits, alpha and beta. The alpha subunit is similar to the alpha subunit found in LH, FSH and TSH glycoprotein hormones. However, the beta subunit is specific and differs from hormone to hormone. The serum hCG rises in early pregnancy to concentrations of 50,000-150,000 mIU/mL between the 8th and 12th weeks of gestation and decline to 20,000 mIU/mL by the 18th week where they remain for the duration of the pregnancy. The increased level of hCG in nonpregnant women or men suggest neoplasia. Thus hCG measurement is useful for the recognition and monitoring of chorionic tumors and as a tumor marker for other malignancies that produce hCG ectopically.
Synonyms: hCG ELISA kit, human Chorionic Gonadotropin ELISA kit.