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CA 19-9 ELISA Kit

CA 19-9 Reaktivität: Human Colorimetric Sandwich ELISA
Produktnummer ABIN930405
  • Target Alle CA 19-9 ELISA Kits anzeigen
    CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
    Reaktivität
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Analytische Methode
    Quantitative
    Produktmerkmale
    ELISA kit for the detection of CA 199 in the research laboratory
    Alternative Names: CA 19-9 ELISA kit
    Top Product
    Discover our top product CA 19-9 ELISA Kit
  • Applikationshinweise
    Optimal conditions to be determined by end user
    Plattentyp
    Pre-coated
    Testdurchführung

    The CA 19-9 ELISA test is based on the principle of a solid phase enzymelinked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA 19-9 molecule is used for solid phase immobilization (on the microtiter wells). Another CA 19-9 monoclonal antibody conjugated to horseradish peroxidase (HRP) is in the antibodyenzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA 19-9 molecules being sandwiched between the solid phase and enzymelinked antibodies. After two separate incubation steps at 37 °C for 90 minutes, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution ch anging the color to yellow. The concentration of CA 19-9 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8 °C.
  • Target Alle CA 19-9 ELISA Kits anzeigen
    CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))
    Andere Bezeichnung
    CA 19-9 (CA 19-9 Produkte)
    Substanzklasse
    Disease
    Hintergrund
    A group of mucin type glycoprotein Sialosyl Lewis Antigens (SLA), such as CA 19-9 and CA 19-5, have come to be recognized as circulating cancer associated antigens for gastrointestinal cancer. CA 19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of CA 19-9 in tissues is consistent with the quantitative determination of higher CA 19-9 concentrations in cancer than in normal or inflamed tissues. Recently reports indicates that the serum CA 19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA, elevated CA 19-9 is suggestive of gall-bladder neoplasm in the setting of inflammatory gallbladder disease. This tumor-associated antigen may also be elevated in some non-malignant conditions. Research studies demonstrate that serum CA 19-9 values may have utility in monitoring subjects with these diagnosed malignancies. It has been shown that a persistent elevation in serum CA 19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum CA 19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA 19-9 value may be indicative of a favorable prognosis and good response to treatment.
    Synonyms: CA 19-9 ELISA kit.
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