Estradiol ELISA Kit

Produktnummer ABIN930631

Produktdetails Estradiol ELISA Kit

Target: Estradiol

Reaktivität: Hormone

Methodentyp: Competition ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of Estradiol in the research laboratory
Alternative Names: Estradiol ELISA kit

Aufreinigung: Antiserum

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Testdurchführung:

This Salivary Estradiol ELISA kit is based on the competition principle and the microplate separation. An unknown amount of Estradiol present in the sample and a fixed amount of Estradiol conjugated with horseradish peroxidase compete for the binding sites of a polyclonal Estradiol antiserum coated onto the wells. After two hours incubation the microtiter plate is washed to stop the competition reaction. Having added the substrate solution the concentration of Estradiol is inversely proportional to the optical density measured.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Referenzen für Estradiol ELISA Kit (ABIN930631)

Song, Condron, Mocca, Veit, Hill, Abbas, Jerse: "Local and humoral immune responses against primary and repeat Neisseria gonorrhoeae genital tract infections of 17beta-estradiol-treated mice." in: Vaccine, Vol. 26, Issue 45, pp. 5741-51, (2008) (PubMed).

Details zu Estradiol

Target: Estradiol

Abstract: Estradiol Produkte

Substanzklasse: Hormone

Hintergrund: Estradiol (1,3,5(10)estratriene3,17 beta-diol, 17 beta-estradiol, E21) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes. Estradiol (E2) is secreted into the blood stream where 98 % of it circulates bound to sex hormone binding globulin (SHBG) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form. Estrogenic activity is effected via estradiol receptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In nonpregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinizing hormone (LH), which are essential for follicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal Estradiol levels increase considerably, to well above the preovulatory peak levels and high levels are sustained throughout pregnancy.
Synonyms: Estradiol ELISA kit.