Histamine ELISA Kit

Produktnummer ABIN930653

Produktdetails Histamine ELISA Kit

Target: Histamine (HIS)

Reaktivität: Chemical

Methodentyp: Competition ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of Histamine in foodstuffs in the research laboratory
Alternative Names: Histamine ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Testdurchführung:

The assay kit provides materials for the quantitative determination of derivatized histamine in food extracts. The derivatization is part of the preparation of the samples. By using the acylation reagent, histamine is derivatized into Nacylhistamine. The competitive Histamine EIA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by antigoat/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Histamine

Target: Histamine (HIS)

Andere Bezeichnung: Histamine (HIS Produkte)

Substanzklasse: Chemical

Hintergrund: Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
Synonyms: Histamine ELISA kit.