Histamine ELISA Kit

Produktnummer ABIN930654

Produktdetails Histamine ELISA Kit

Target: Histamine (HIS)

Reaktivität: Ratte

Methodentyp: Competition ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of Rat Histamine in the research laboratory
Alternative Names: Rat Histamine ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Testdurchführung:

During the sample preparation Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Histamine

Target: Histamine (HIS)

Andere Bezeichnung: Histamine (HIS Produkte)

Substanzklasse: Chemical

Hintergrund: Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
Synonyms: Rat Histamine ELISA kit.