Histamine ELISA Kit

Produktnummer ABIN930656

Produktdetails Histamine ELISA Kit

Target: Histamine (HIS)

Reaktivität: Chemical

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of Histamine release from heparinized whole blood in the research laboratory
Alternative Names: Histamine ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Testdurchführung:

Heparinized whole blood samples are incubated with different concentrations of the suspected allergen. Release of histamine will occur upon stimulation of basophilic granulocytes depending on their sensibility to the allergen. The released histamine in the supernatant is subsequently determined using a specific plasma immunoassay for the detection of histamine purchased in connection with this kit. This histamine value is related to the 100 % control (= Total Histamine) and the blank value (= Spontaneous Release). The determination of the in vitro release of histamine represents a sensitive and specific method as well as a suitable addition to routine diagnostic procedures including conventional skin testing and radioallergosorbent tests (RAST) for the determination of specific IgE anbodies in serum of atopic patients. In addition, this test also detects the releasability of the cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Histamine

Target: Histamine (HIS)

Andere Bezeichnung: Histamine (HIS Produkte)

Substanzklasse: Chemical

Hintergrund: Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
Synonyms: Histamine ELISA kit.