Dopamine ELISA Kit

Produktnummer ABIN930670

Produktdetails Dopamine ELISA Kit

Target: Dopamine (DA)

Reaktivität: Chemical

Methodentyp: Competition ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of Dopamine in the research laboratory
Alternative Names: Dopamine ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Testdurchführung:

Dopamine is extracted by using a cisdiolspecific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Dopamine

Target: Dopamine (DA)

Andere Bezeichnung: Dopamine (DA Produkte)

Substanzklasse: Chemical

Hintergrund: Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptor (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
Synonyms: Dopamine ELISA kit.