Thyroperoxidase ELISA Kit

Produktnummer ABIN930685

Produktdetails Thyroperoxidase ELISA Kit

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for the detection of Thyroid peroxidase in the research laboratory
Alternative Names: Thyroid peroxidase ELISA kit, TPO ELISA kit, MSA ELISA kit, TPX ELISA kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end-user

Plattentyp: Pre-coated

Testdurchführung:

Highly purified human thyroid peroxidase (TPO) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu Thyroperoxidase

Target: Thyroperoxidase (TPO) (Thyroid Peroxidase (TPO))

Andere Bezeichnung: Thyroid peroxidase (TPO Produkte)

Hintergrund: Thyroid peroxidase or thyroperoxidase (TPO) is an enzyme expressed mainly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine (T4) or triiodothyronine (T3), thyroid hormones. In humans, thyroperoxidase is encoded by the TPO gene.
Synonyms: Thyroid peroxidase ELISA kit, TPO ELISA kit, MSA ELISA kit, TPX ELISA kit.

Pathways: Thyroid Hormone Synthesis