GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting

Produktnummer ABIN965412

Hersteller Produkt- Nr.: kca215

Produktdetails

Applikation: Dot Blot (DB), Western Blotting (WB)

Hersteller Produkt- Nr.: kca215

Hersteller: Rockland

Kreuzreaktivität: recombinant GFP (rGFP), enhanced GFP (eGFP)

Anwendungsinformationen

Applikationshinweise: GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Kommentare:

Detection Kit Type: Chemiluminescent Western Blot Kit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Lagerung: RT/4 °C

Antigendetails

Hintergrund: GFP Chemiluminescent Western Blot Kit allows for the detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: GFP Western Blotting Kit, GFP Chemiluminescent Kit, Green Fluorescent Protein Antibody Kit, Immunoblotting Kit, GFP, rGFP, eGFP