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Cell ELISA Kits

Der "Zell-ELISA", auch bekannt als In-Cell-ELISA, zellbasierter ELISA oder In-Cell Western, ist eine quantitative immunzytochemische Methode zur Messung von Proteinniveaus oder posttranslationalen Modifikationen von kultivierten Zellen. Er stellt eine einfache, empfindliche Methode zur intrazellulären Proteinquantifizierung in ganzen Zellen dar. Zell-ELISA eignen sich hervorragend zur Überwachung von Effekten der Stimulation und/oder Hemmung von Behandlungen, Verbindungen (z.B. siRNA, Chemikalien) auf Zielmoleküle.

Der Zell-ELISA bietet als Methode einige entscheidende Vorteile. Die Messung des antigenen Zielmoleküls in lebenden kultivierten Zellen negiert jegliche Interferenz oder verwirrende Wirkung der Verwendung von harten Lysereagenzien. Darüber hinaus ist die In-Zell-Methode schneller, skalierbarer und hat einen höheren Durchsatz als andere vergleichbare proteomische Methoden wie Western Blotting und/oder Sandwich-ELISA. Zell-ELISA sind für den täglichen Gebrauch bequem und effizient. Zellbasierte ELISA-Kits erfordern lebende, adhärente Zellen für die Messung und sind daher nicht für die Messung von Standardprobentypen wie Plasma, Serum oder Gewebekulturmedien geeignet. Stattdessen sollten Forscher, die diese Probenarten untersuchen wollen, einen herkömmlichen Sandwich- oder kompetitiven ELISA wählen.

Ergebnisse filtern:

Alle Filter

1.469 Treffer :

ERK1/2 ELISA Kit

MAPK1/3 Reaktivität: Human, Ratte, Maus pThr202 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentrations of EGF for 10 min at 37

5 Abbildungen

10 Referenzen

Produktnummer ABIN1981830

Datenblatt (PDF)

JNK ELISA Kit

MAPK8 Reaktivität: Human, Maus, Ratte pThr183 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

Hela cells were stimulated by different concentrations of anisomycin for 15 minutes at 37 °C

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

6 Abbildungen

4 Referenzen

Produktnummer ABIN1981833

Datenblatt (PDF)

STAT1 ELISA Kit

STAT1 Reaktivität: Human, Maus, Ratte pTyr701 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C.

Example of how to seed cells for cell-based assay

A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37 °C

6 Abbildungen

2 Referenzen

Produktnummer ABIN1981837

Datenblatt (PDF)

STAT3 ELISA Kit

STAT3 Reaktivität: Human, Ratte, Maus pTyr705 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431cells.

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration EGF for 30 min at 37 °C

6 Abbildungen

3 Referenzen

Produktnummer ABIN1981839

Datenblatt (PDF)

STAT5A ELISA Kit

STAT5A Reaktivität: Human, Ratte, Maus pTyr694 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37 °C

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C

6 Abbildungen

1 reference

Produktnummer ABIN1981843

Datenblatt (PDF)

MAPK14 ELISA Kit

MAPK14 Reaktivität: Human, Ratte, Maus pThr180 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

This picture shows the reagent preparation.

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

Cell-Based protein phosphorylation procedure

Hela cells were stimulated by different concentrations of anisomycin for 15 minutes at 37 °C

Example of how to seed cells for cell-based assay

6 Abbildungen

4 Referenzen

Produktnummer ABIN1981835

Datenblatt (PDF)

STAT1,STAT3,STAT5 ELISA Kit

Reaktivität: Human, Maus, Ratte pTyr705 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431cells.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration EGF for 30 min at 37 °C

This picture shows the reagent preparation.

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C.

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

A431 cells were stimulated by different concentrat ions of EGF for 30 minutes at 37 °C

7 Abbildungen

4 Referenzen

Produktnummer ABIN1981847

Datenblatt (PDF)

ERK1/2, JNK, p38 MAPK ELISA Kit

Reaktivität: Human, Maus, Ratte pThr183 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

Cell-Based protein phosphorylation procedure

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C.

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

This picture shows the reagent preparation.

9 Abbildungen

6 Referenzen

Produktnummer ABIN1981832

Datenblatt (PDF)

STAT6 ELISA Kit

STAT6 Reaktivität: Human, Ratte, Maus pTyr641 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration of recombinant human EGF for 10 min at 37 °C

5 Abbildungen

1 reference

Produktnummer ABIN1981845

Datenblatt (PDF)

STAT4 ELISA Kit

STAT4 Reaktivität: Human, Maus, Ratte pTyr693 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Cell-Based protein phosphorylation procedure

Image no. 3 for Signal Transducer and Activator of Transcription 4 (STAT4) ELISA Kit (ABIN1981841)

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

5 Abbildungen

3 Referenzen

Produktnummer ABIN1981841

Datenblatt (PDF)

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