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Cell ELISA Kits

Der "Zell-ELISA", auch bekannt als In-Cell-ELISA, zellbasierter ELISA oder In-Cell Western, ist eine quantitative immunzytochemische Methode zur Messung von Proteinniveaus oder posttranslationalen Modifikationen von kultivierten Zellen. Er stellt eine einfache, empfindliche Methode zur intrazellulären Proteinquantifizierung in ganzen Zellen dar. Zell-ELISA eignen sich hervorragend zur Überwachung von Effekten der Stimulation und/oder Hemmung von Behandlungen, Verbindungen (z.B. siRNA, Chemikalien) auf Zielmoleküle.

Der Zell-ELISA bietet als Methode einige entscheidende Vorteile. Die Messung des antigenen Zielmoleküls in lebenden kultivierten Zellen negiert jegliche Interferenz oder verwirrende Wirkung der Verwendung von harten Lysereagenzien. Darüber hinaus ist die In-Zell-Methode schneller, skalierbarer und hat einen höheren Durchsatz als andere vergleichbare proteomische Methoden wie Western Blotting und/oder Sandwich-ELISA. Zell-ELISA sind für den täglichen Gebrauch bequem und effizient. Zellbasierte ELISA-Kits erfordern lebende, adhärente Zellen für die Messung und sind daher nicht für die Messung von Standardprobentypen wie Plasma, Serum oder Gewebekulturmedien geeignet. Stattdessen sollten Forscher, die diese Probenarten untersuchen wollen, einen herkömmlichen Sandwich- oder kompetitiven ELISA wählen.

Ergebnisse filtern:

Alle Filter

1.469 Treffer :

STAT6 ELISA Kit

STAT6 Reaktivität: Human, Ratte, Maus pTyr641 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration of recombinant human EGF for 10 min at 37 °C

5 Abbildungen

1 reference

Produktnummer ABIN1981845

Datenblatt (PDF)

STAT4 ELISA Kit

STAT4 Reaktivität: Human, Maus, Ratte pTyr693 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Cell-Based protein phosphorylation procedure

Image no. 3 for Signal Transducer and Activator of Transcription 4 (STAT4) ELISA Kit (ABIN1981841)

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

5 Abbildungen

3 Referenzen

Produktnummer ABIN1981841

Datenblatt (PDF)

BrdU ELISA Kit

BrdU Colorimetric Cell ELISA Cell Culture Cells

ELISA image for Bromodeoxyuridine (BrdU) ELISA Kit (ABIN955803)

ELISA image for Bromodeoxyuridine (BrdU) ELISA Kit (ABIN955803)

ELISA image for Bromodeoxyuridine (BrdU) ELISA Kit (ABIN955803)

Flow Cytometry (FACS) image for Bromodeoxyuridine (BrdU) ELISA Kit (ABIN955803)

ELISA image for Bromodeoxyuridine (BrdU) ELISA Kit (ABIN955803)

ELISA image for Bromodeoxyuridine (BrdU) ELISA Kit (ABIN955803)

6 Abbildungen

Produktnummer ABIN955803

Datenblatt (PDF)

EGFR ELISA Kit

EGFR Reaktivität: Human, Maus, Ratte pTyr992 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

A431 cells were stimulated by different concentrations of EGF for 20 min at 37 °C

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C

Example of how to seed cells for cell-based assay

Cell-Based protein phosphorylation procedure

This picture shows the reagent preparation.

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C

11 Abbildungen

Produktnummer ABIN1981829

Datenblatt (PDF)

Tyrosine ELISA Kit

Reaktivität: Human, Maus, Ratte phosphorylated Colorimetric Cell ELISA Cell Culture Cells

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were treated for 30 min with 50 µL of 100 mM Genistein or 2 mM Lavendustin in appropriate wells at room temperature prior to EGF stimulation.

Example of how to seed cells for cell-based assay

4 Abbildungen

5 Referenzen

Produktnummer ABIN1981848

Datenblatt (PDF)

EGFR ELISA Kit

EGFR Reaktivität: Human phosphorylated Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

A431 cells were stimulated by different concentrations of EGF for 20 min at 37 °C

Cell-Based protein phosphorylation procedure

4 Abbildungen

4 Referenzen

Produktnummer ABIN1981821

Datenblatt (PDF)

8isoPGF2a ELISA Kit

8isoPGF2a Reaktivität: Others Colorimetric Cell ELISA Plasma, Serum

Dilutions of Human Urine Tested with the OxiSelect™ 8-iso-Prostaglandin F2alpha ELISA Kit.

1 image

32 Referenzen

Produktnummer ABIN2344990

Datenblatt (PDF)

Cellular UV-Induced DNA Damage ELISA Kit

Reaktivität: Others Colorimetric Cell ELISA Cell Samples

DNA Damage Induced by UV Light in Hela Cells.

DNA Damage Induced by UV Light in HeLa Cells.

2 Abbildungen

5 Referenzen

Produktnummer ABIN2344977

Datenblatt (PDF)

CytoSelect™ BrdU Cell Proliferation ELISA Kit

ELISA, ProA Reaktivität: Säugetier Colorimetric Cell Samples Uncoated

Serum Stimulation of Proliferation in HEK 293 Cells.

1 image

3 Referenzen

Produktnummer ABIN2344922

Datenblatt (PDF)

TUBG1 ELISA Kit

TUBG1 Reaktivität: Human, Maus, Ratte Colorimetric Cell ELISA Cell Culture Cells

1 reference

Produktnummer ABIN1381574

Datenblatt (PDF)

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