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Lhca4 Antikörper

Lhca4 Reaktivität: Spinach, Mais (Zea mays), Arabidopsis thaliana, Gerste, Moss, Nicotiana tabacum, Oryza sativa WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN93514
  • Target
    Lhca4 (psi Type IV Chlorophyll A/B-Binding Protein (Lhca4))
    Reaktivität
    Spinach, Mais (Zea mays), Arabidopsis thaliana, Gerste, Moss, Nicotiana tabacum, Oryza sativa
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Kreuzreaktivität (Details)
    peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 22 / 21 kDa for Arabidopsis thaliana
    Aufreinigung
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.
    Isotyp
    IgG
  • Applikationshinweise
    Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

    Albus, Salinas, Czarnecki, Kahlau, Rothbart, Thiele, Lein, Bock, Grimm, Schöttler: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).

  • Target
    Lhca4 (psi Type IV Chlorophyll A/B-Binding Protein (Lhca4))
    Hintergrund
    AGI Code: At3g47470
    The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.
    Molekulargewicht
    expected: 22 kDa, apparent: 21 kDa for Arabidopsis thaliana
    UniProt
    P27521
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