DYKDDDDK Positive Control Lysate
PC, WB
Whole Cell Lysate
Overexpression Lysate
E. coli
Reaktivität: Tag
Produktnummer ABIN1607004
Hersteller Produkt- Nr.: w00-001-383
Kurzübersicht für DYKDDDDK Positive Control Lysate (ABIN1607004)
Target
DYKDDDDK Tag
Proteinspezies
Tag
Applikation
Positive Control (PC), Western Blotting (WB)
Lysed Cells
E. coli
Lysate Type
Overexpression Lysate
Lysate Fraction
Whole Cell Lysate
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Lysatspezies
- Escherichia coli (E. coli)
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Hersteller Produkt- Nr.
- w00-001-383
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Hersteller
- Rockland
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Endotoxin-Niveau
- Low Endotoxin : No
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Applikationshinweise
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
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Lagerung
- -80 °C
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Informationen zur Lagerung
- Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
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Haltbarkeit
- Expiration date is three (3) months from date of opening.
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- DYKDDDDK Tag
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Substanzklasse
- Tag
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Hintergrund
- This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
Target
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