Adipose Subcutaneous Diabetic Disease Lysate
-
- Lysatspezies
- Human
- Applikation
- Western Blotting (WB)
- Produktmerkmale
-
Human subcutaneous adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The human subcutaneous adipose tissue total protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the subcutaneous adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The subcutaneous adipose tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Source: Human Subcutaneous Adipose tissue
Diagnosis: Diseased
Tissue or Cell type: Adipose - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
-
VNN1 293T Cell Transient Overexpression Lysate(Denatured)
VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
BIRC3 293T Cell Transient Overexpression Lysate(Denatured)BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human
Hep2 Whole Cell LysateWB Whole Cell Lysate Cell Lysate HepG2 Cells Reaktivität: Human
NIH/3T3 Whole Cell Lysate (PDGF Stimulated)WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reaktivität: Maus
DYKDDDDK Positive Control LysatePC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Tag
A431 Whole Cell Lysate (EGF Stimulated)WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reaktivität: Human
CD177 (human): 293T LysateCD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
-
- Applikationshinweise
- Adipose Subcutaneous lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 2 mg/mL
- Buffer
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- Handhabung
- Repeated freezing and thawing is not recommended.
- Lagerung
- 4 °C/-80 °C
- Informationen zur Lagerung
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- Haltbarkeit
- 6 months
-
