Liver Tissue Lysate (Tumor)
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- Lysatspezies
- Human
- Applikation
- Western Blotting (WB)
- Spezifität
- Liver Tissue
- Produktmerkmale
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Liver tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1 % Triton X-100, 1 % sodium deoxycholic acid, 0.1 % sodium dodecylsulfate, 5 μ,g/mL of aprotinin, 5 μ,g/mL of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5 % glycerol, 1 % sodium dodecylsulfate, 0.01 % bromophenol blue) containing 5 % β,-mercaptoethanol.
Source: Human Liver Tissue
Age: 64
Gender: F
Race: Caucasian
Diagnosis: Tumor
Cancer Type: Trabecular pattern hepatocellular carcinoma
- Lysate Type
- Tumor Lysate
- Lysate Fraction
- Whole Tissue Lysate
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 2 mg/mL
- Buffer
- SDS sample buffer containing 5 % β,-mercaptoethanol.
- Handhabung
- Repeated freezing and thawing is not recommended.
- Lagerung
- 4 °C/-80 °C
- Informationen zur Lagerung
- Store at 2-8˚,C for continuous use. For extended storage, freeze working aliquots at -70˚,C.
- Haltbarkeit
- 6 months
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