Proteinspezies: Maus
Lysatspezies: Maus
Applikation: Western Blotting (WB)
Spezifität: Tissues were washed exhaustively with PBS to remove blood and other debris. A lysate was prepared by homogenizing the tissue and washing the cells in cold PBS. Washed cells were incubated at 4° C in modified RIPA buffer containing 150 mM sodium chloride, 50 mM Tris Cl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid and 0.1% SDS to lyse the cells. Protein integrity is ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 µM Aprotinin, 5 µM Bestatin, 1.5 µM E-64, 2 µM Leupeptin Hemisulfate and 1 µM Pepstatin A). The following phosphatase inhibitors were also added: 1 mM NaF and 1 mM Na3VO4. Cell debris was removed by centrifugation and membrane filtration. Protein concentration was determined by Lowry assay using a commercially available kit. The protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.
Produktmerkmale: Cell Line: Primary tissue
Induction: None (Control)
Sterilität: Sterile filtered
Lysate Type: Tissue Lysate
Lysate Fraction: Whole Tissue Lysate

VNN1 293T Cell Transient Overexpression Lysate(Denatured)

VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)

CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

APLP2 293T Cell Transient Overexpression Lysate(Denatured)

APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

BIRC3 293T Cell Transient Overexpression Lysate(Denatured)

BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

BAG1 293T Cell Transient Overexpression Lysate(Denatured)

BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Human

Hep2 Whole Cell Lysate

WB Whole Cell Lysate Cell Lysate HepG2 Cells Reaktivität: Human

NIH/3T3 Whole Cell Lysate (PDGF Stimulated)

WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reaktivität: Maus

DYKDDDDK Positive Control Lysate

PC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reaktivität: Tag

A431 Whole Cell Lysate (EGF Stimulated)

WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reaktivität: Human

CD177 (human): 293T Lysate

CD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells

Applikationshinweise: Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
Kommentare: Lysate Fractionation: Whole Cell Lysate
Lysate Stimulation: Not Stimulated
Lysate Tissue Culture: Primary Tissue
Beschränkungen: Nur für Forschungszwecke einsetzbar

Format: Liquid
Konzentration: 2.0 mg/mL
Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Handhabung: Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 °C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µl depending on the size format of your gel.
Lagerung: -80 °C
Haltbarkeit: 3 months

Hintergrund: Ready-to-use whole cell lysates produced are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Normal Maus Kidney Whole Cell Lysate

Produktdetails