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MSP2N2 Protein (His tag)

MSP2N2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % STB
Produktnummer ABIN3199238
  • Target
    MSP2N2 (Nanodisc Membrane Scaffold Protein 2N2 (MSP2N2))
    Protein-Typ
    Recombinant
    Spezies
    Human
    Quelle
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses MSP2N2 Protein ist gelabelt mit His tag.
    Applikation
    Stabilization (STB)
    Verwendungszweck
    Forms nanodisc structures in combination with phospholipids
    Spezifität
    Stabilization of membrane proteins
    Produktmerkmale
    • MSP2N2 protein, human sequence, corresponds to duplicated MSP1D1 protein
    • N-terminal 6x His-tag
    • Resulting nanodisc diameter: ca. 15-17 nm

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Reinheit
    > 90 %
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Bestandteile
    Lyophilized MSP protein
    Benötigtes Material
    • 37 °C incubator
    • 4 °C incubator
    • End-over-end shaker
    • Micropipettor and Micropipetting tips
    • Gel filtration column
    • FPLC instrument with integrated UV detector and fraction collector
    • Magnetic stirrer
    • Centrifuge for 2 mL microtubes
    • 2 mL microtubes
    • Palmitoyl-oleoyl-phosphatidylcholine (POPC)
    • Dimyristoyl-glycero-phosphocholine (DMPC) or other suitable phospholipid or phospholipid mixture
    • Sodium cholate
    • Biobeads SM-2
    • Single use syringe
    • Single use needle
    • 0.45 micron filter
    • Protein concentrator
    • SDS PAGE reagents and equipment

    • Optional: Western Blot reagents and equipment and PentaHis Antibody
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Sample Volume for an assay: >1 mg purified membrane protein In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Protokoll
    Stabilization of membrane proteins using nanodiscs
    Ergebnisberechnung

    SDS-PAGE / Western Blot

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Lagerung
    -20 °C
  • Target
    MSP2N2 (Nanodisc Membrane Scaffold Protein 2N2 (MSP2N2))
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