The spleen cells from mouse, immunized with recombinant protein of human type ISR-A, were fused with mouse NS-1 myeloma cells. The hybridoma cell line withpositive reaction was grown in ascitic fluid of BALB/c mouse, from which theantibody was purified by Protein G affinity chromatography.
Immunogen
recombinant protein of human type I SR-A
Klon
SRA-E5
Isotyp
IgG1
Applikationshinweise
Working dilution for immunohistochemistry: 10ug/ml , for immunoblotting : 2ug/mlAntigen retrieval (microwave 10 min , 0.01M citrate buffer, ph2.0) is recommended for paraffin sections.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.25 mg/ml
Buffer
PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)
Konservierungsmittel
ProClin
Lagerung
-20 °C
Target
MSRA
(Macrophage Scavenger Receptor A (MSRA))
Andere Bezeichnung
Macrophage Scavenger Receptor A(MSR-A:CD204)
Hintergrund
Macrophage scavenger receptor (MSR-A: CD204) was identified in the search for the receptor molecules thatare implicated in the pathological deposition of cholesterol during atherogenesis through receptor-mediateduptake of modified low density lipoproteins (LDL).MSR-As possess a wide range of ligand-binding specificities and recognize a variety of molecules such asmodified LDL including acetylated LDL, oxidized LDL, advanced glycation end products (AGE),polyribonucleotides such as poly G and poly I and bacterial surface lipids including lipopolysaccharide andlipoteicoic acid.This antidody was produced from the mouse immunized with recombinant protein of human type IMSR-A ,and has been proved to be useful for the immunoblotting and immunohistochemistry.This antibody is useful tools for the study of MSR in atherogenesis and various other pathological conditionsin humans and animal species.