Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) Antikörper
Verified
NR1H2/3
Reaktivität: Human
ELISA, FACS, IF, WB
Wirt: Ziege
Polyclonal
unconjugated
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- Target Alle Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) Produkte
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
- Bindungsspezifität
- AA 429-442, AA 443-456
- Reaktivität
- Human
- Wirt
- Ziege
- Klonalität
- Polyklonal
- Applikation
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
- Verwendungszweck
- LXR alpha / LXR beta
- Sequenz
- CRLQDKKLPP LLSEI
- Spezifität
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
- Kreuzreaktivität
- Rind (Kuh), Hund, Human, Maus, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Immunogen
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
- Isotyp
- IgG
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- Applikationshinweise
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Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
- Kommentare
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
- Andere Bezeichnung
- NR1H3, NR1H2
- Synonyme
- LXR-a antikoerper, LXRA antikoerper, RLD-1 antikoerper, nuclear receptor subfamily 1 group H member 3 antikoerper, NR1H3 antikoerper
- Hintergrund
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
- Gen-ID
- 10062, 7376
- NCBI Accession
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
- Pathways
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
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