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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Peptid

AKT1 Wirt: Synthetic WB Without preservative
Rockland
Produktnummer ABIN1043545
Hersteller Produkt- Nr.: 000-000-260
  • Target Alle AKT1 Produkte
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    Protein-Typ
    Synthetic
    Quelle
    • 26
    Synthetic
    Applikation
    Western Blotting (WB)
    Hersteller Produkt- Nr.
    000-000-260
    Hersteller
    Rockland
    Verwendungszweck
    Substrate Peptide AKT/PKBa
    Produktmerkmale
    AKT1, serine/threonine kinase, apoptosis inhibitor substrate peptides, blocking peptide, control peptide
    Reinheit
    Greater than 98% specific peptide by HPLC analysis
  • Applikationshinweise

    Application Note: AKT/PKBa Substrate control peptide should be used at 1.0 μg per 1.0 μL of antiserum in per assay.

    Western Blot Dilution: 1:500 - 1:2,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Reconstitution Volume: 500 μL

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: None

    Stabilizer: None

    Preservative: None
    Konservierungsmittel
    Without preservative
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 2 - 8 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
    Haltbarkeit
    6 months
  • Target
    AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
    Hintergrund
    Background: Akt is a serine/threonine kinase that enhances cell proliferation and inhibits apoptosis when activated. Akt associates with PI(3,4,5)P3) at the cell membrane through its pleckstrin homology domain and is activated through phosphorylation. 3-Phosphoinositide-dependent kinase-1 (PDK1) has been shown to first phosphorylate Akt at Thr308, then a second kinase phosphorylates Akt at position Ser473. In turn, Akt phosphorylates a host of proteins including the proapoptotic proteins BAD and pro-caspase-9, GSK3, p21WAF1, MDM2, and the forkhead (FKHR) family of transcription factors.
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