A working concentration of 500 μg/mL for triple DYKDDDDK peptide is typically used for competitive elution of DYKDDDDK containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK DYKDDDDK affinity gel PROTOCOL for recommendations for use.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Konzentration
1 mg/mL
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2 - 8 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Haltbarkeit
Expiration date is six (6) months from date of opening.
Target
DYKDDDDK Tag
Substanzklasse
Tag
Hintergrund
The Triple DYKDDDDK Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic DYKDDDDK sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK). The triple DYKDDDDK peptide is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free triple DYKDDDDK Peptide. Synonyms: synthetic DYKDDDDK triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide