When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
NCKAP1L
(NCK-Associated Protein 1-Like (NCKAP1L))
Synonyme
HEM1 Peptide, 4930568P13Rik Peptide, AI463083 Peptide, Hem1 Peptide, Hemp1 Peptide, NCK associated protein 1 like Peptide, zgc:172352 Peptide, nck-associated protein 1-like Peptide, NCK-associated protein 1-like Peptide, NCKAP1L Peptide, zgc:172352 Peptide, LOC100030968 Peptide, nckap1l Peptide, LOC100618382 Peptide, LOC100639088 Peptide, Nckap1l Peptide