When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
STAP2
(Signal Transducing Adaptor Family Member 2 (STAP2))
Synonyme
BKS Peptide, AW049765 Peptide, STAP-2 Peptide, wu:fa55b07 Peptide, zgc:77021 Peptide, cb901 Peptide, stap2 Peptide, wu:fd15d07 Peptide, zgc:63913 Peptide, signal transducing adaptor family member 2 Peptide, signal transducing adaptor family member 2b Peptide, signal transducing adaptor family member 2a Peptide, STAP2 Peptide, Stap2 Peptide, stap2b Peptide, stap2a Peptide