When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
RASSF1
(Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))
Synonyme
RASSF1 Peptide, zgc:92505 Peptide, wu:fc17f02 Peptide, zgc:112224 Peptide, 123F2 Peptide, NORE2A Peptide, RASSF1A Peptide, RDA32 Peptide, REH3P21 Peptide, AA536941 Peptide, AU044980 Peptide, Rassf1A Peptide, Rassf1B Peptide, Rassf1C Peptide, NPR2-like, GATOR1 complex subunit Peptide, Ras association (RalGDS/AF-6) domain family 1 Peptide, Ras association domain family member 1 Peptide, putative rassf1 Peptide, Ras association domain family member 1 L homeolog Peptide, Ras association (RalGDS/AF-6) domain family member 1 Peptide, NPRL2 Peptide, rassf1 Peptide, RASSF1 Peptide, Smp_157340 Peptide, rassf1.L Peptide, Rassf1 Peptide