When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
PIAS2
(Protein Inhibitor of Activated STAT, 2 (PIAS2))
Synonyme
MGC83751 Peptide, piasx Peptide, wu:fi05f09 Peptide, PIAS2 Peptide, 6330408K17Rik Peptide, AI462206 Peptide, ARIP3 Peptide, AU018068 Peptide, Dib Peptide, Miz1 Peptide, PIASxalpha Peptide, PIASxb Peptide, PIASxbeta Peptide, SIZ2 Peptide, DIP Peptide, MIZ Peptide, MIZ1 Peptide, PIASX Peptide, PIASX-ALPHA Peptide, PIASX-BETA Peptide, ZMIZ4 Peptide, protein inhibitor of activated STAT 2 L homeolog Peptide, protein inhibitor of activated STAT 2 Peptide, protein inhibitor of activated STAT, 2 Peptide, pias2.L Peptide, PIAS2 Peptide, pias2 Peptide, Pias2 Peptide