When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
RPS27
(Ribosomal Protein S27 (RPS27))
Synonyme
MPS-1 Peptide, MPS1 Peptide, S27 Peptide, 3200001M24Rik Peptide, GB13072 Peptide, DDBDRAFT_0203425 Peptide, DDBDRAFT_0231065 Peptide, DDB_0203425 Peptide, DDB_0231065 Peptide, mps-1 Peptide, mps1 Peptide, ribosomal protein S27 Peptide, 40S ribosomal protein S27 Peptide, ribosomal protein S27 pseudogene Peptide, Ribosomal protein S27, component of cytosolic 80S ribosome and 40S small subunit Peptide, component of 80S ribosome and 40S small subunit Peptide, ribosomal protein S27 L homeolog Peptide, RPS27 Peptide, Rps27 Peptide, LOC726295 Peptide, RPS27P Peptide, rps27 Peptide, rps27.L Peptide, rps-27 Peptide