When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Lagerung
-20 °C
Informationen zur Lagerung
Shipped at ambient temperature, store at -20°C.
Target
SET/TAF-I (SET)
(SET Nuclear Oncogene (SET))
Synonyme
2PP2A Peptide, I2PP2A Peptide, IGAAD Peptide, IPP2A2 Peptide, PHAPII Peptide, TAF-I Peptide, TAF-IBETA Peptide, 2610030F17Rik Peptide, 5730420M11Rik Peptide, AA407739 Peptide, I-2PP2A Peptide, StF-IT-1 Peptide, 2pp2a Peptide, i2pp2a Peptide, igaad Peptide, ipp2a2 Peptide, phapii Peptide, set Peptide, set-a Peptide, set-b Peptide, taf-ibeta Peptide, wu:fb30g11 Peptide, wu:fd16c08 Peptide, SET nuclear proto-oncogene Peptide, SET nuclear oncogene Peptide, SET translocation Peptide, SET nuclear proto-oncogene L homeolog Peptide, SET nuclear proto-oncogene b Peptide, SET Peptide, Set Peptide, LOC403555 Peptide, set.L Peptide, setb Peptide