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Potassium Channel KAT3 Antikörper

KAT3 Reaktivität: Arabidopsis thaliana WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN488529
  • Target
    Potassium Channel KAT3 (KAT3)
    Reaktivität
    Arabidopsis thaliana
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Kreuzreaktivität (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Homologie
    Arabidopsis thaliana
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 75.5 / 75 kDa
    Aufreinigung
    serum
    Immunogen
    KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
  • Applikationshinweise
    1: 50 with 125I (WB)
    Kommentare

    In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 100 μL of sterile water.
    Handhabung
    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    Potassium Channel KAT3 (KAT3)
    Andere Bezeichnung
    KC1
    Synonyme
    A. thaliana low-K+-tolerant 1 antikoerper, ARABIDOPSIS THALIANA K+ RECTIFYING CHANNEL 1 antikoerper, ATKC1 antikoerper, AtLKT1 antikoerper, F4D11.150 antikoerper, F4D11_150 antikoerper, K+ INWARD RECTIFYING CHANNEL PROTEIN antikoerper, KC1 antikoerper, potassium channel in Arabidopsis thaliana 3 antikoerper, potassium channel protein antikoerper, KAT3 antikoerper
    Hintergrund
    AGI Code: At4g32650
    KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
    Molekulargewicht
    expected: 75.5 kDa, apparent: 75 kDa
    UniProt
    P92960
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