Protein A Protein (HRP)

Produktnummer ABIN1044879

Produktdetails

Target: Protein A

Spezies: Staphylococcus aureus

Wirt: Bitte anfragen

Aufreinigungstag / Konjugat: Dieses Protein A Protein ist gelabelt mit HRP.

Applikation: ELISA, Western Blotting (WB)

Spezifität: Protein A Peroxidase was prepared from chromatographically pure Protein A. Protein A Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.

Anwendungsinformationen

Applikationshinweise: Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

Kommentare:

Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: Protein A

Abstract: Protein A Produkte

Synonyme: alpha protein Protein, repB Protein

Hintergrund: Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Synonyms: HRP, peroxidase, Horseradish peroxidase protein A