Recombinant Human Anterior Gradient Protein 3 Homolog/AG-3 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Thr22-Leu166) of Human AGR3 fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
AGR3
Spezies: Maus
Wirt: Human Cells
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
ELISA, WB, SDS, MS
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 2 mM EDTA, pH 8.5.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Sub Type
Fusionprotein
Hintergrund
Anterior Gradient Protein 2(AG-2) and Anterior Gradient Protein 3 (AG-3) are human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan (hAG-3 protein). AG-3 could serve as a prognostic marker for survival in patients with low grade and high grade serous ovarian carcinomas. Alternative Names: Anterior Gradient Protein 3 Homolog, AG-3, AG3, hAG-3, Breast Cancer Membrane Protein 11, AGR3, BCMP11