Recombinant Human C-Type Lectin Domain Family 4 Member E/CLEC4E is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Arg41-Leu219) of Human CLEC4E fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
CLEC4E
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, ELISA, WB
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Haltbarkeit
3 months
Target
CLEC4E
(C-Type Lectin Domain Family 4, Member E (CLEC4E))
CLECSF9 Protein, MINCLE Protein, C86253 Protein, Clecsf9 Protein, Mincle Protein, CLEC4E Protein, C-type lectin domain family 4 member E Protein, C-type lectin domain family 4, member e Protein, C-type lectin domain family 4, member E Protein, CLEC4E Protein, Clec4e Protein, LOC611538 Protein
Sub Type
Fusionprotein
Hintergrund
C-Type Lectin Domain Family 4 Member E (CLEC4E) is a 219 amino acid single-pass type II membrane protein that contains one C-type Lectin domain. It is expressed in monocytes, CLEC4E functions as a downstream target of C/EBP beta and is thought to play a role in the inflammatory response, possibly via transcriptional control of C/EBP beta. CLEC4E may play a role in the response to inflammatory stimuli in peritoneal macrophages and may be involved in immune surveillance processes under transcriptional control of CEBPB. Human CLEC4E shares 67% sequence identity with its mouse counterpart, suggesting a similar function between species. CLEC-4E exists as multiple alternatively spliced isoforms that are encoded by a gene which maps to a natural killer gene complex region on human chromosome 12. Alternative Names: C-Type Lectin Domain Family 4 Member E, C-Type Lectin Superfamily Member 9, Macrophage-Inducible C-Type Lectin, CLEC4E, CLECSF9, MINCLE