FEN1 Protein (AA 1-380)

Produktnummer ABIN1096675

Produktdetails

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-380

Spezies: Human

Quelle: Escherichia coli (E. coli)

Verwendungszweck: Recombinant Human Flap Endonuclease 1/FEN-1

Sequenz: MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK

Produktmerkmale: Recombinant Human Flap Endonuclease 1/FEN-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys380) of Human FEN-1.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, 1 mM DDT, 10 % Glycerol, pH 8.0.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: -80 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: flap-endonuclease-1 (FEN1 Produkte)

Synonyme: FEN-1 Protein, FEN1 Protein, AW538437 Protein, MF1 Protein, RAD2 Protein, cb879 Protein, fen-1 Protein, fen1 Protein, mf1 Protein, rad2 Protein, xFEN1a Protein, flap structure-specific endonuclease 1 Protein, flap endonuclease 1-like Protein, Flap endonuclease-1 Protein, 5'-3' exonuclease family protein Protein, flap structure specific endonuclease 1 Protein, flap structure-specific endonuclease 1 L homeolog Protein, nuclease, Rad2 family Protein, FEN1 Protein, LOC100808044 Protein, Bm1_49605 Protein, AT5G26680 Protein, Fen1 Protein, fen1 Protein, fen1.L Protein

Hintergrund: Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Alternative Names: Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-Specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, FEN1, RAD2

Molekulargewicht: 42.59 kDa

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA