Recombinant Human Interleukin-1alpha/IL-1alpha produced in E. coli is a single non-glycosylated polypeptide chain containing 159 amino acids with a molecular mass of 18 kDa.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
IL1A
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Interleukin-1 alpha (IL1alpha) is a cytokine member of the interleukin-1 family. IL-1 consists of two distinct forms: IL1alpha and IL1beta that recognize the same cell surface receptors but are distinct proteins with approximately 25% amino acid sequence identity. IL1alpha is constitutively produced by epithelial cells and plays an essential role in maintenance of skin barrier function. Upon stimulation, a wide variety of cells including osteoblasts, monocytes, macrophages can be induced to express IL1alpha. IL1alpha possesses a wide range of metabolic, physiological, haematopoietic activities, and is critically involved in the regulation of the immune responses and inflammatory responses. Alternative Names: Interleukin-1 Alpha, IL-1 Alpha, Hematopoietin-1, IL1A, IL1F1