Recombinant Human Low-Density Lipoprotein Receptor/LDLR is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala22-Arg788) of Human LDLR fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
LDLR
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
LDLR
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by SDS-PAGE
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.4.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
FH Protein, FHC Protein, LDLCQ2 Protein, Hlb301 Protein, LDLRA Protein, LDLA Protein, LDL receptor-2 Protein, fhc Protein, ldlcq2 Protein, ldlr Protein, ldlr2-a Protein, LDL receptor 1 Protein, ldlr-a Protein, ldlr-b Protein, low density lipoprotein receptor Protein, low density lipoprotein receptor a Protein, low density lipoprotein receptor S homeolog Protein, low density lipoprotein receptor L homeolog Protein, LDLR Protein, Ldlr Protein, ldlra Protein, ldlr.S Protein, ldlr.L Protein
Sub Type
Fusionprotein
Hintergrund
Low-Density Lipoprotein Receptor (LDLR) is a transmembrane glycoprotein that plays a critical role in cholesterol homeostasis. LDLR mediates blood cholesterol level by interacting with lipoprotein particles like LDL and VLDL. The extracellular domain of LDLR contains LDL receptor type A (ligand-binding) modules (LA repeats), epidermal growth factor-like modules, and LY repeats containing the YWTD consensus motif that are important in binding and releasing of ApoB-100 and ApoE in lipoprotein particles. The C terminal domain of LDLR inside the cell is required for the receptor internalization. Loss of function mutations in the LDLR gene causes Familial Hypercholesterolemia (FH). Alternative Names: Low-Density Lipoprotein Receptor, LDL Receptor, LDLR