Recombinant Human Serpin A4 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gln21-Pro427) of Human SERPINA4 fused with a polyhistidine tag at the C-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
SERPINA4
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 10 mM NaCl, pH 8.0.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Haltbarkeit
3 months
Target
SERPINA4
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))
KLKR Protein, Klk6 Protein, hK1 Protein, KAL Protein, KLST Protein, KST Protein, PI-4 Protein, PI4 Protein, kallistatin Protein, SERPINA4 Protein, DKFZp469E1737 Protein, KALps Protein, Klst Protein, kallikrein 1 Protein, serpin family A member 4 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 Protein, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 Protein, KLK1 Protein, SERPINA4 Protein, Serpina4 Protein, SPIA4 Protein, Serpina4-ps1 Protein
Sub Type
Fusionprotein
Hintergrund
Serpin Peptidase Inhibitor, Clade A (alpha-1 Antiproteinase, Antitrypsin), Member 4 (Serpin A4) is a member of the Serpin family. Serpin A4 exists as a monomer and some homodimers. Serpin A4 is expressed by the liver and secreted in plasma. Serpin A4 is a regulator of vascular homeostasis capable of controlling a wide spectrum of biological actions in the cardiovascular and renal systems. It can inhibit intracellular reactive oxygen species formation in cultured cardiac and renal cells. In addition, Serpin A4 has anti-inflammatory effect. Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity. Alternative Names: Kallistatin, Kallikrein Inhibitor, Peptidase Inhibitor 4, PI-4, Serpin A4, SERPINA4, KST, PI4