MDH protein (His tag)
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- Target
- MDH
- Protein-Typ
- Recombinant
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Spezies
- E. coli
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- His tag
- Applikation
- SDS-PAGE (SDS)
- Aufreinigung
- purified by chromatography
- Reinheit
- > 95 % by SDS - PAGE
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-
- Kommentare
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Synonyms: malate dehydrogenase, ECK3225, JW3205
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Avoid repeated freezing and thawing cycles.
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- Target
- MDH
- Synonyme
- MDH Protein, MMDH Protein, malate dehydrogenase 2 Protein, MDH2 Protein
- Hintergrund
- Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molekulargewicht
- 34.9kDa (336aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_417703
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