Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (His tag)

Produktnummer ABIN1098613

Produktdetails

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Hi-5 Cells

Aufreinigungstag / Konjugat: Dieses Glucose-6-Phosphate Dehydrogenase Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by using conventional chromatography.

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: glucose-6-phosphate 1-dehydrogenase, G6PD1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 MM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: G6PD (G6PD Produkte)

Hintergrund: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Molekulargewicht: 61.4kDa (535aa)

NCBI Accession: NP_001035810

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones